Syllabus Application
Experimental Techniques in Biology
BIO 335
Faculty:
Faculty of Engineering and Natural Sciences
Semester:
Fall 2025-2026
Course:
Experimental Techniques in Biology - BIO 335
Classroom:
FENS-L058,FENS-L065
Level of course:
Undergraduate
Course Credits:
SU Credit:3.000, ECTS:6, Basic:5, Engineering:1
Prerequisites:
-
Corequisites:
BIO 335L
Course Type:
Lecture
Instructor(s) Information
Ali Rıza Umut Şahin
- Email: umut.sahin@sabanciuniv.edu
Course Information
Catalog Course Description
A general introduction to basic techniques used in characterization and separation of biological systems and molecules. Topics include microscopy, centrifugation, liquid chromatography methods, spectroscopy and electrophoresis. Lab sessions will give a chance to use these techniques individually.
Course Learning Outcomes:
| 1. | Upon completion of the course, students will have a general knowledge of basic laboratory techniques for characterization and separation of biological systems and molecules. Students would be able to perform basic laboratory techniques. |
|---|---|
| 2. | Module 1 Microscopy: Students will have a deeper understanding of both, theory and application in the following topics: Basic Concepts in Microscopy Light microscopy Fluorescence microscopy Confocal Microscopy Live-cell imaging and Sensor techniques |
| 3. | Module 2 Electrophoresis Techniques: Students will learn how to use Gel electrophoresis techniques for analysis of nucleic acids and proteins for both, diagnostic purposes and preparative purposes, which includes: Basic Concepts in Electrophoresis Horizontal and Vertical Electrophoresis 2D Gel Electrophoresis and Protein Detection Methods Electrophoresis of Nucleic Acids |
| 4. | Module 3 Centrifugation Techniques: Basic Concepts of Centrifugation Techniques Types of Centrifuges and analytical ultracentrifugation techniques Separation methods and preparative ultracentrifuges Types of rotors |
| 5. | Module 4 Spectroscopic Techniques: Introduction and basic concepts of Spectroscopic Techniques UV-VIS Spectroscopy Infrared and fluorescence spectroscopy Circular dichroism (CD) spectroscopy NMR Spectroscopy and X-ray crystallography Atomic Spectroscopy and Mass spectroscopy |
| 6. | Module 5 PCR Techniques: Principles of Polymerase Chain reaction Preparative and Diagnostic PCR DNA Sequencing methods Sanger Sequencing, Next Generation Sequencing Methods |
| 7. | Module 6 Chromatography and Mass spectrometry: Basic Principles of Chromatography Thin layer chromatography Liquid Chromatography Gas Chromatography Principles of MALDI-TOF Electrospray Ionization Mass Spectrometry |
Course Objective
To teach students the basic concepts of bioanalytical techniques in both, theory and practice, which are relevant to biologists and bioengineers but also students from different fields.